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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Nutr Food Res. 2015 Mar 17;59(5):991–999. doi: 10.1002/mnfr.201400761

Figure 2. Zinc deficiency induced macrophage-like cell activation and increased proinflammatory response in THP1 cells.

Figure 2

THP1 cells were cultured in ZA or ZD media for up to 3 weeks, followed by LPS stimulation (100 ng/ml) for 24h (n=3–4 per group). Cell surface expression of activation markers ICAM1, HLA-DR, and CD86 were analyzed by flow cytometry. (A) Representative flow cytometry histograms showing ICAM1 expression in untreated and LPS-treated ZA and ZD THP1 cells. (B) ICAM1, (C) HLA-DR, and (D) CD86 cell surface expression in THP1 cells cultured in ZA media, or ZD media for 1, 2, and 3 weeks followed by 24h LPS treatment (100 ng/ml) (black bars). Control cells were left untreated (white bars). (E) IL1β and (F) IL6 proinflammatory cytokines production in culture supernatant in LPS-treated (gray bars) and untreated (white bars) ZA and ZD THP1 cells. Data represent mean ± SEM. Results are representative of three independent experiments. MFI, mean fluorescence intensity.

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