Figure 3. Zinc deficiency resulted in decreased IL6 promoter methylation.
(A) IL1β and (B) IL6 proinflammatory cytokines production in ZA THP1 cells left untreated, or treated with 5 µM AZA for 48h prior to LPS stimulation (100 ng/ml) for 24h (n=3 per group). Data represent mean ± SEM. *P<0.01 vs. ZA. Results are representative of two independent experiments. (C) IL6 promoter methylation in ZA, ZD, and AZA-treated THP1 cells (n=5–9 per group). Data represent mean promoter methylation fold-change ± SEM vs. ZA. Means without a common letter differ, P < 0.05. (D) Global DNA methylation in ZA, ZD (3 wk), and AZA-treated THP1 cells (n=4 per group). Data represents mean relative fluorescence units (RFU) ± SEM per 200 ng DNA. Means without a common letter differ, P < 0.05. Results are representative of two independent experiments.