Figure 4. Aging was associated with increased ICAM1 and IL6 gene expression and decreased IL6 promoter methylation.
(A) Plasma zinc concentrations in young (2 mo) and aged (26 mo) mice (n=6 per group). Data represent mean ± SEM. *P < 0.05 vs. young. (B) ICAM1 and IL6 gene expression in the spleens of young (2mo) and aged (26mo) mice (n=6 per group). Data represent mean normalized fold-change ± SEM vs. young. *P < 0.01 vs. young. (C) IL6 promoter methylation in the spleens of young (2mo) and aged (26mo) mice (n=6 per group). Data represent mean methylation percentage ± SEM. *P < 0.01 vs. young. (D) IL6 promoter methylation in human lymphoblastic cell lines derived from young (20–28 yr) and aged (92–101 yr) individuals represented as box and whiskers plot (n=10 per group). Whiskers represent maximum and minimum values.