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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: Nat Cell Biol. 2006 Aug 20;8(9):1017–1024. doi: 10.1038/ncb1464

Figure 2.

Figure 2

Expression of CALM–AF10 is sufficient to cause mouse bone-marrow cell transformation and hDOT1L has an important role in this process. (a) Schematic representation of the retroviral transduction procedures described in the Methods. (b) Confirmation of expression of the transduced genes by RT–PCR. U937 cells serve as a positive control. GAPDH serves as a control for equal input of RNA in RT–PCR. (c) Serial colony-plating assay of bone-marrow cells transduced by wild-type and mutant CALM–AF10 with vector control. The average colony numbers ± s.d. of three independent experiments are shown. The P value of the third round colony numbers between the wild-type and the OM–LZ deletion mutant is also indicated. (d) Growth curve of the transduced cells in mFTOC culture. (e) Morphology of colonies formed in the third round of methylcellulose assays transduced by CALM–AF10. The scale bar represents 0.5 mm. Wright-Giemsa-stained cytospin preparation of cells from the third round of colonies is also shown. The scale bar represents 50 μm. (f) Immunophenotyping by FACS of CALM–AF10 transformed cells. The cell surface markers used in the analysis are indicated. (g) Schematic representation of the second transduction procedure and the effect of wild-type and catalytic-deficient hDOT1L on colony formation of CALM–AF10 transformed cells. RV, retrovirus.