Skip to main content
. 2015 Jan 12;24(10):1223–1235. doi: 10.1089/scd.2014.0176

FIG. 5.

FIG. 5.

Expression of chemokine receptors and of proteins involved in EM degradation after incubation with Huh-7 CM. MSC isolated from four different donors have been treated with control condition (CTRL), PDGF-BB, and Huh-7 CM for 24 h. (a) Total RNA were extracted, and real-time PCR was performed on CCR1, CCR6, and CXCR2. (b) Real-time PCR was performed for MMP-1, MMP-2, and TIMP-1. All experimental data are mean±SD from at least three independent experiments (**P<0.02). (c) MSC were seeded at a density of 30,000 cells per well in a 24-well plate. Cells were incubated with control medium (CTRL), control medium containing MIP-1δ and MIP-3α, as well as Huh-7 CM. Western blotting were performed using an anti-MMP1 antibody. GAPDH was used as loading control. This figure shows one representative experiment out of three. (d) The graph presents intensity of signals that was evaluated by analyzing the optical density of the spots. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. n.s., no significance.