Table 18.
DNA vaccines for fish.
| Pathogen | Species | Vaccine vector |
Dosage | Vaccination age |
Protection | Conventional treatment |
Ref. |
|---|---|---|---|---|---|---|---|
| IHNV(Apex-IHN by Novartis, UK) |
Atlantic salmon |
G-protein gene |
Two 20-µg doses im. |
30 g or larger | 73% protection | Avoiding exposure to the virus |
[208] |
| IPNV(Mikalsen et al. 2004) |
Atlantic salmon |
VP2 gene | 25 µg of DNA im. |
Postmolts of 20 g or larger |
RPS* of 84% | Commercial vaccines: inactivated virus or structural virus proteins expressed in Escherichia coli |
[124] |
| VHSV (Lorenzen et al. 2001) |
Rainbow trout |
G-protein gene |
1 µg of DNA im. |
Fry 0.5 g (3 months after hatching) |
RPS of 98% | [125] | |
|
Mycobacterium marinum (Pasnik and Smith 2004) |
Striped bass |
AG85A gene (secreted fibronectin- binding protein) |
25 or 50 µg doses with booster 14 days later im. |
40–50 g (~5 months) | Low levels of AG85A- specific antibodies, lymphoproliferative responses within 42 days, 80% RPS for 25-µg dose, 90% for 50-µg dose |
No chemotherapeutic agents; avoiding exposure to bacteria |
[126] |
*
Relative percent survival (= one cumulative mortality vaccinated group/cumulative mortality control group).
im.: Intramuscular; RPS: Relative percent survival.