The F-BAR Protein Cdc15 Serves as a Primary Mitotic Anchor for the Actomyosin in S. japonicus
(A) Time-lapse color-composite maximum-projection images of wild-type (top), cdc15-A temperature-sensitive mutant (middle), and cdc15-Amid1Δ double-mutant (bottom) S. japonicus cells expressing Rlc1-GFP and Pcp1-mCherry after incubation at indicated temperatures for 1 hr. Time is in minutes.
(B) Kymographs showing dynamics of Cdc15-GFP and Rlc1-mCherry in mitotic wild-type (top) and mid1Δ (bottom) S. japonicus cells. Time interval is 10 s.
Scale bars represent 5 μm. See also Figure S2.