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. 2015 May 4;25(9):1187–1194. doi: 10.1016/j.cub.2015.02.056

Figure 3.

Figure 3

S. japonicus Cells Lacking the Polarisome Kinase Pom1 Misposition the Division Site

(A) Calcofluor staining of wild-type and pom1Δ S. japonicus cells. Plot (right) shows proportion of cells exhibiting division septa at various positions along the long cell axis. 150 cells were counted for each genotype.

(B) Maximum-projection images of interphase wild-type (top) and pom1Δ (bottom) S. japonicus cells expressing Rlc1-GFP and LifeAct-mCherry, Mid1-GFP, and Cdc15-GFP. The monopolar growth pattern in pom1Δ cells is revealed by LifeAct-mCherry.

(C) Time-lapse maximum-projection images of wild-type (left) and pom1Δ (right) S. japonicus cells co-expressing Rlc1-GFP and Nhp6-mCherry.

(D) A montage of time-lapse maximum-projection images of pom1Δmid1Δ S. japonicus cells co-expressing Rlc1-GFP and Nhp6-mCherry.

(E) Time-lapse maximum-projection images of wild-type (top) and pom1Δmid1Δ (bottom) S. japonicus cells co-expressing Cdc15-GFP and Pcp1-mCherry (n = 6 cells). Dashed lines indicate cell boundaries.

For (C)–(E), time is in minutes. Scale bars represent 5 μm. See also Figure S3.