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. 2015 May 4;33(3):285–298. doi: 10.1016/j.devcel.2015.02.017

Figure 6.

Figure 6

The N Terminus of Nup1 Is Required for NPC and NE Integrity

(A) Double-fluorescence microscopy of cells expressing the indicated Nup1 mCherry constructs (endogenous promoter) in nup1Δ cells harboring different genomically GFP-tagged Nups. White arrows label cytoplasmically mislocalized Nup82/159. Note that some cells also contain cytoplasmic NLSnup1Δ1-123 foci. Scale bar represents 2 μm. Appending an NLS to full-length Nup1 does not result in a similar mislocalization pattern (see Figure S7A).

(B) TEM analysis of exponentially growing cells after 4hr treatment with 0.2% benzyl alcohol. The indicated Nup1 constructs (endogenous promoter) were expressed in nup1Δ cells. Inset shows higher magnification of a membrane herniation (black arrow). White arrows indicate NPCs. Scale bar represents 500 nm (100 nm for inset).