Figure 5.

sFlaF Conserved Residues and Function

(A) The conserved residues chosen for further mutation analysis are depicted in a ball and stick model.

(B) Motility assay of complemented S. acidocaldarius strains. To check the importance of the conserved residues, the residues were mutated to alanine on a complementation plasmid and ΔaapFΔflaF strains were transformed and spotted on motility plates. The S. acidocaldarius ΔaapFΔflaI deletion mutant was complemented using either wild-type flaI (FlaI^WT) as a positive control and E336A flaI (FlaI^WB) as a negative control. The figure represents one of the three biological replicas of the motility assay and the numbers below each strain depict the percentage of the relative diameter of the swimming halo compared with the positive control from three individual biological samples. The black ring indicates the growth zone, whereas the white ring indicates the motility zone.