Table 2. Proteins identified in reproduction-related glands of Apis mellifera drones by SEQUEST and de novo approach.
| Uniprot | RefSeq | BeeBase | Description | Function | Peptides (DB/Total) | B | C | V |
|---|---|---|---|---|---|---|---|---|
| A5A5E4 | - | GB46297-PA | Structural cuticle protein (Fragment) OS = Apis mellifera PE = 2 SV = 1 | Structural molecule activity | 8/20 | X | X | |
| H9KBS5 | NP_001257759 | GB52161-PA | PREDICTED: flexible cuticle protein 12 OS = Apis mellifera GN = CPR28 PE = 4 SV = 1 | Structural molecule activity | 5/8 | X | X | |
| H9KGM8 | - | GB46297-PA | PREDICTED: endocuticle structural glycoprotein SgAbd-2-like OS = Apis mellifera | Structural molecule activity | 17/17 | X | ||
| H9K0P8 | XP_001120021 | GB44777-PA | PREDICTED: hypothetical protein LOC725882 OS = Apis mellifera GN = LOC725882 PE = 4 | Molybdopterin biosynthesis protein MoeB domain; Validated | 1/1 | X | ||
| H9KU41 | - | GB46312-PA | PREDICTED: endocuticle structural glycoprotein SgAbd-8 OS = Apis mellifera | Structural molecule activity | 8/11 | X | X | |
| Q6VQ13 | NP_001010975 | GB42422-PA | ADP/ATP translocase OS = Apis mellifera GN = Ant PE = 2 SV = 1 | Transporter activity, cytoplasm, membrane, mitochondrial | 5/8 | X | X | |
| H9KHD2 | XP_392478 | GB42526-PA | PREDICTED: malate dehydrogenase, mitochondrial-like isoform1 OS = Apis mellifera | Carbohydrate metabolism, citric acid cycle | 2/2 | X | ||
| H9KC10 | XP_001120194 | GB45913-PA | PREDICTED: protein lethal(2)essential for life-like OS = Apis mellifera GN = LOC724488 PE = 3 | Heat-shock protein 20 (HSP20) | 1/1 | X | ||
| H9KJ51 | - | GB55598-PA | Troponin I OS = Apis mellifera PE = 4 SV = 1 | Muscle constriction, part of troponin complex, binds actin | 1/1 | X | ||
| H9KA48 | - | GB40759-PA | Icarpin precursor OS = Apis mellifera PE = 4 SV = 1 | Venom carbohydrate-rich protein, secreted by venom duct, extracelular | 1/2 | X | ||
| H9KMZ3 | XP_625049 | GB48244-PA | Cyclin-dependent kinase 6-like OS = Apis mellifera PE = 4 SV = 1 | Kinase activity, phosphorylation, cell cycle regulation | 1/1 | X | ||
| H9KTW5 | XP_624662 | GB48904-PA | PREDICTED: glutathione S-transferase-like OS = Apis mellifera GN = LOC552283 PE = 3 | Transferase, processing of xenobiotics, detoxification, diverse functions | 1/1 | X | ||
| H9K5H8 | XP_392114 | GB51551-PA | PREDICTED: myophilin OS = Apis mellifera GN = Chd64 PE = 4 SV = 1 | Muscle associated | 1/1 | X | ||
| H9K6R4 | XP_001121551 | GB46094-PA | PREDICTED: actin, cytoplasmic 1-like, partial OS = Apis mellifera GN = LOC725739 PE = 4 | Muscle constriction | 1/1 | X | ||
| H9K538 | XP_393297 | GB42792-PA | PREDICTED: hypothetical protein LOC409805 OS = Apis mellifera GN = Mf PE = 4 SV = 1 | Unknown | 2/3 | X | ||
| H9KIT9 | XP_624806 | GB43999-PA | PREDICTED: peroxiredoxin-5, mitochondrial OS = Apis mellifera GN = Prx5 PE = 4 SV = 1 | Response to oxidative stress, cell redox homeostasis | 2/2 | X | X | |
| B0LUE8 | NP_001107670 | GB55452-PA | Apolipophorin-III-like protein OS = Apis mellifera GN = A4 PE = 2 SV = 1 | Lipid transport, extracelular | 1/1 | X | ||
| H9KMZ2 | - | - | PREDICTED: hypothetical protein LOC552453 OS = Apis mellifera GN = LOC552453 PE = 4 | Conserved Domain: Protein of unknown function (DUF745); Drosophila proteins family | 1/1 | X | ||
| H9K1E2 | XP_006559246 | GB54446-PA | Arginine kinase OS = Apis mellifera GN = Argk PE = 3 SV = 1 | Phosphorylation, arginine metabolism, proline metabolism | 1/4 | X | X | |
| H9KKD2 | XP_001121746 | GB53748-PA | PREDICTED: hypothetical protein LOC725960 OS = Apis mellifera GN = LOC725960 PE = 4 | Unknown | 1/1 | X | ||
| H9K7H5 | XP_001122696 | GB42797-PA | PREDICTED: protein takeout-like OS = Apis mellifera GN = LOC726981 PE = 4 SV = 1 | Probably: chemoreception; Probably: circadian-controled | 1/1 | X | ||
| H9KGE6 | XP_393789 | GB47268-PA | PREDICTED: electron transfer flavoprotein subunit beta-like OS = Apis mellifera | Phosphorylation, electron transport, microtubule associated, lipid particle | 1/1 | X | ||
| Q5XUU6 | NP_001011640 | GB48492-PA | Take-out-like carrier protein JHBP-1 OS = Apis mellifera PE = 2 SV = 1 | Transfer of Juvenile Hormone PFAM: Pf06585 | 1/1 | X | ||
| H9KR59 | XP_006568820 | GB55482-PA | PREDICTED: Na(+)/H(+) exchange regulatory cofactor NHE-RF1-like OS = Apis mellifera | PDZ signaling domain: protein-protein interaction, signaling | 1/1 | X | ||
| H9KP88 | - | GB54676-PA | Aspartate aminotransferase OS = Apis mellifera GN = Ame.2149 PE = 3 SV = 1 | Aminoacid metabolism, alkaloid synthesis | 1/1 | X | ||
| H9K024 | XP_006567025 | GB52107-PA | Tubulin alpha OS = Apis mellifera GN = LOC408388 PE = 3 SV = 1 | Microtubule comp., protein polymeryzation, antimicrobial humoral response | 1/1 | X | ||
| H9K626 | XP_393545 | GB52073-PA | PREDICTED: probable citrate synthase 1, mitochondrial-like OS = Apis mellifera | Carbohydrate metabolism, citric acid cycle | 1/1 | X | ||
| H9KNB7 | XP_003251464 | GB41306-PA | Actin OS = Apis mellifera GN = LOC410075 PE = 3 SV = 1 | Muscle constriction | 1/3 | X | ||
| H9KPL4 | XP_392899 | GB54372-PA | PREDICTED: 60 kDa heat shock protein, mitochondrial-like OS = Apis mellifera | Response to stress, protein refolding, transmembrane transport, pH regulation | 1/3 | X | ||
| H9K918 | XP_006564892 | GB52736-PA | Probably: ATP synthase subunit beta OS = Apis mellifera GN = Atp5b PE = 3 SV = 1 | Oxidative phosporylation, proton transport, ATP synthesis | 1/1 | X | ||
| H9KR70 | XP_625171 | GB53437-PA | PREDICTED: f-box only protein 32-like OS = Apis mellifera PE = 4 SV = 1 | Cell cycle regulation, signal transduction | 1/1 | X | ||
| H9KUC2 | NP_001257753 | GB52202-PA | Cuticular protein 12 precursor [Apis mellifera] | Structural molecule activity | 0/1 | X | ||
| H9K3K6 | XP_623787 | GB50875-PA | PREDICTED: apolipoprotein D-like isoform 2 [Apis mellifera] | Lipid transport, lipid binding, pigment binding | 0/1 | X | ||
| H9KCL3 | - | GB53775-PA | PREDICTED: retinol dehydrogenase 14-like [Apis mellifera] | Oxidoreductase activity | 0/1 | X | ||
| H9K639 | - | GB47336-PA | PREDICTED: protein disulfide-isomerase [Apis mellifera] | Protein folding, disulfide formation. Location: extracellular, EPR | 0/1 | X | ||
| H9JYV0 | - | GB53065-PA | PREDICTED: 60S ribosomal protein L7 [Apis mellifera] | Strctural constituent of ribosome | 0/1 | X | ||
| H9KEY1 | XP_392760 | GB55643-PA | PREDICTED: ATP synthase subunit O, mitochondrial [Apis mellifera] | ATP synthesis, pfam00213 | 0/3 | X | X | X |
| H9KAU3 | XP_624910 | GB54343-PA | PREDICTED: 10 kDa heat shock protein, mitochondrial-like [Apis mellifera] | Response to stress, protein folding | 0/4 | X | X | |
| H9K748 H9KU35 | - | GB51653-PA | PREDICTED: myosin heavy chain, muscle [Apis mellifera] Also: H9KU34, H9KU33, H9KU31 | Motor activity, actin binding | 0/5 | X | ||
| H9KUH8 H9KLF7 | XP_006559096 | GB51800-PA | PREDICTED: hypothetical protein LOC409090 [Apis mellifera] | ATPase inhibitor domain, metabolic process regulation, pfam04568, pfam13873 | 0/1 | X | ||
| H9KHG2 | XP_001121882 | GB52644-PA | PREDICTED: ATP synthase-coupling factor 6, mitochondrial [Apis mellifera] | Mitochondrial proton-transporting ATP synthase complex | 0/1 | X | ||
| H9KN88 | - | GB40460-PA | PREDICTED: probable enoyl-CoA hydratase, mitochondrial [Apis mellifera] | Fatty acid beta-oxidation, fatty acid metabolism | 0/1 | X | ||
| H9K4S1 | XP_393900 | GB48255-PA | PREDICTED: isochorismatase domain-containing protein 1-like [Apis mellifera] | Hydrolase activity, metabolic process | 0/1 | X | ||
| H6CSZ2 H9KBB1 | - | GB49545-PA | Glutathione S-transferase S4 [Apis mellifera] Also: B6VCW8 | Transferase activity | 0/2 | X | ||
| H9K8G6 | XP_006558384 | GB40240-PA | PREDICTED: myosin regulatory light chain 2 [Apis mellifera] | Ca-binding, superoxide metabolism, oxidation-reduction process | 0/1 | X | ||
| H9KPS8 | XP_624401 | GB50595-PA | PREDICTED: aldo-keto reductase, NADP+ dependent [Apis mellifera] | Oxidoreductase activity | 0/1 | X | ||
| H9K0L2 | XP_393300 | GB48536-PA | PREDICTED: t-complex protein 1 subunit beta-like isoform 1 [Apis mellifera] | Protein folding, Chaperone | 0/1 | X | ||
| H9KR11 | XP_006563323 | GB40735-PA | PREDICTED: fructose-bisphosphate aldolase-like [Apis mellifera] | Glycolysis | 0/1 | X | ||
| H9K9A4 | XP_001120471 | GB55232-PA | PREDICTED: 3-hydroxyacyl-CoA dehydrogenase type-2-like [Apis mellifera] | Oxidoreductase activity, dehydrogenase-reductase | 0/1 | X |
Uniprot accession number, functional description, number of peptides supporting the protein found during database step (DB) and database + de novo step (Total) and the origin are provided for each protein. Proteins received additional support during de novo step are marked with bold font, proteins found only during de novo step are marked with bold-italic font. B—bulbus gland, C—cornua gland, V—vesicula seminalis.