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. 2015 May 8;10(5):e0126445. doi: 10.1371/journal.pone.0126445

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© 2015 Kimura et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A, B) Brain-restricted activation of dsx-GAL4 neurons induced mating-type extrusion of the ovipositor in a female with the genotype of UAS>stop>dTrpA1-myc/Otd-nsl:FLP; dsx ^GAL4 (G) / + by an increase in temperature to 32°C. The ventral view (A) and lateral view (B) of a mated female displaying mating-type extrusion are shown. (C, D) Activation of dsx-GAL4 neurons outside the brain induced oviposition-type extension/egg ejection in a female with the genotype of Tub>stop>GAL80/Otd-nsl:FLP; dsx ^GAL4 (G) UAS- dTrpA1 / + by an increase in temperature to 32°C. The ventral view (C) and lateral view (D) of a mated female displaying oviposition-type extension are shown. (E) The proportion of females that exhibited mating-type extrusion/egg-laying (left) or oviposition-type extension/egg-laying (right) upon a temperature increase to 32°C. The numbers shown in parentheses (E) indicate the number of flies examined. The drawings below the graph are nervous systems and the red colored region represents the activating sites by dTRPA1.