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. 2015 May 8;10(5):e0126279. doi: 10.1371/journal.pone.0126279

Fig 1. Immunization protocol.

Fig 1

Mice were immunized i.p. with untreated BLG (n = 15) or OVM (n = 15) three times, followed by two oral challenges (OCs) on days 42 and 43. Subsequently, mice were divided in three groups per allergen (n = 5) and challenged i.v. with vehicle control on day 49 followed by i.v allergen challenges on day of sacrifice. Blood samples were collected on days 42, 49 and 56. BLG, beta-lactoglobulin; i.v., intravenous; OC, oral challenge; OVM, ovomucoid