Fig 2. Induction of allergen specific IgE, IgA, IgG1 and IgG2a in immunized mice.

Immunization elicited equally elevated specific IgE titers in mice being injected with the same allergen (A, BLG; B, OVM) whereas naïve control groups did not show any allergen specific IgE antibody production. BLG (C, E, G) and OVM (D, F, H) allergic mice showed significantly higher levels of allergen specific IgG1, IgG2a and IgA compared with naïve mice. Significantly lower levels of specific IgG2a were observed in the group i.v. challenged with nitrated BLG (BLGn). Significantly lower levels of specific IgG1 were found in the group i.v. challenged with nitrated OVM (OVMn). Independent of the isotype, higher antibody levels were found in mice sensitized with BLG compared to OVM sensitized animals. Data obtained for IgG2a of OVM allergic mice were transformed by applying the logarithm to base 10. Antibody levels of groups were compared at the time-point of sacrifice with One-way ANOVA followed by Tukey post test. (**P<0.01, ***P<0.001) BLG, beta-lactoglobulin; BLGn, nitrated BLG; i.v., intravenous; OVM, ovomucoid; OVMn, nitrated OVM