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. 2015 May 8;10(5):e0126648. doi: 10.1371/journal.pone.0126648

Fig 4. Expression vectors for wild-type (WT) and the four allelic variants with bTSH promoter.

Fig 4

Plasmids expressing the various LHX4 mutants were transiently cotransfected into heterologous HEK293T cells with a luciferase reporter gene under the control of the bTSH promoter. Promoter activity was assayed by measuring luciferase activity 48h after transfection. Negative controls (control) received equivalent amounts of empty expression vector plasmid. Results are expressed as fold-increase of luciferase signal relative to control. *p<0.05.