Skip to main content
. 2015 May 8;10(5):e0125937. doi: 10.1371/journal.pone.0125937

Fig 7. Co-immunoprecipitation of CsmA-HA with mRFP-KinArigor or GFP-KinA and FLAG-ChsB with mRFP-KinArigor in vivo.

Fig 7

(A, B) 100,000 xg pellets (P100) of cell extracts of the strains expressing CsmA-HA, mRFP-KinArigor, or both of them (A), and the strains expressing CsmA-HA, GFP-KinA, or both of them (B) were immunoprecipitated with anti-HA antibody. P100 (Input) and immunoprecipitates with anti-HA antibody (IP: α-HA) were subjected to Western blot analysis using antibodies indicated by IB. (C) Those of the strains expressing FLAG-ChsB or FLAG-ChsB and mRFP-KinArigor were immunoprecipitated with anti-FLAG antibody and subjected to Western blot analysis.