Table 1. A. nidulans strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
| ABPU1 | biA1 pyrG89 argB2 pyroA4 wA3 | [19] |
| A1149 | pyrG89 pyroA4 ΔnkuA::argB | FGSC* |
| CsmA-HA/FLAG-ChsB1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::csmA-9xHA-pyrG ΔargB::argB-chsB(p)-3xFLAG-chsB | This study |
| EGFP-CsmA1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-egfp-csmA | This study |
| EB-5 | biA1 pyrG89 argB2 pyroA4 wA3 ΔchsB::pyr4-alcA(p)-chsB ΔargB::argB-chsB(p)-egfp-chsB | [24] |
| SNR1 | ΔargB::trpΔB ΔkinA::pyr4 pyrG89 pyroA4 yA2 | [45] |
| SNZ9 | pyrG89 argB2 ΔnkuA::argB pyroA4 ΔuncA::pyroA | [48] |
| EGFP-CsmAΔkinA1, 2 | ΔargB::trpCΔB ΔkinA::pyr4 pyrG89 pyroA4 yA2 ΔcsmA::argB-alcA(p)-egfp-csmA | This study |
| EGFP-CsmAΔuncA1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-egfp-csmA ΔuncA::pyroA | This study |
| EGFP-ChsBΔkinA1, 2 | ΔargB::trpCΔB ΔkinA::pyr4 pyrG89 pyroA4 yA2 ΔargB::argB-chsB(p)-egfp-chsB | This study |
| EGFP-ChsBΔuncA1, 2 | argB2 pyroA4 wA3 ΔargB::argB-chsB(p)-egfp-chsB ΔuncA::pyroA | This study |
| EGFP-CsmA/mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-egfp-csmA ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| EGFP-ChsB/mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔchsB::pyr4-alcA(p)-chsB ΔargB::argB-chsB(p)-egfp-chsB ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| ABPU1/mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| CA2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::csmA-9xHA-pyrG | [61] |
| FB-3 | biA1 pyrG89 argB2 pyroA4 wA3 ΔchsB::pyr4-alcA(p)-chsB ΔargB::argB-chsB(p)-3XFLAG-chsB | [24] |
| CsmA-HA/mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::csmA-9HA-pyrG ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| FLAG-ChsB/ mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔchsB::pyr4-alcA(p)-chsB ΔargB::argB-chsB(p)-3XFLAG-chsB ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| EGFP-ΔMA1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-egfp-csmA(Δ1–860) | This study |
| EGFP-ΔMA/ mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-egfp-csmA(Δ1–860) ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| CΔMHA | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-csmA(Δ1–860)-9xHA-pyrG | [25] |
| D10HA | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-csmA(Δ347–356)-9xHA-pyrG | [25] |
| CΔMHA/ mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-csmA(Δ1–860)-9xHA-pyrG ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| D10HA/ mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::argB-alcA(p)-csmA(Δ347–356)-9xHA-pyrG ΔkinA::pyroA-alcA(p)-mrfp-kinA rigor | This study |
| ABPU1/GFP-KinA1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔkinA::pyroA-alcA(p)-gfp-kinA | This study |
| CsmA-HA/GFP-KinA1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔkinA::pyroA-alcA(p)-gfp-kinA ΔcsmA::pyrG-csmA-9HA | This study |
| CsmA-HA/FLAG-ChsB/ mRFP-KinArigor1, 2 | biA1 pyrG89 argB2 pyroA4 wA3 ΔcsmA::csmA-9xHA-pyrG argB::chsB(p)-3xFLAG-chsB ΔkinA::pyroA4-alcA(p)-mrfp-kinA rigor | This study |
*: Fungal Genetics Stock Center, Kansas City, Missouri