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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2015 Mar 18;30:46–52. doi: 10.1016/j.dnarep.2015.03.004

Fig. 1.

Fig. 1

Effects of Top1 knockdown on cell division and proliferation. For ΦV− AIDR2 cells, 3 sh-control and 9 sh-Top1 clones were tested, and for AID+/+ cells, 2 sh-control and 4 sh-Top1 clones were tested. (A) Western blot analysis comparing Top1 levels in sh-control and sh-Top1 clones. Representative blots are shown. Bar graphs represent the average Top1/β-actin ratio; error bars depict standard deviation (SD). P value, unpaired two-tailed Student’s t test. (B) Cell division experiments tracking the dilution of CFSE dye over time. Representative flow cytometry analysis of fluorescence intensity in a sh-control clone on days 0 (black), 1 (green), and 2 (orange). Bar graphs represent the average mean intensity; error bars indicate SD. Value for d 1 includes cells in both peaks. (C) Cell proliferation. Cells were counted on days 0–4.