Fig 4. Non-synonymous substitutions in the deduced protein of duck EDNRB2.

(A) Schematic diagram representing duck EDNRB2. Prediction of the secondary protein structure was obtained using online website ExPASy (http://www.expasy.org/vg/index/Protein). The extracellular and intracellular loops are shown as white boxes. The seven transmembrane domains are indicated as green boxes. Non-synonymous mutations identified in chicken [13], Japanese quail [14] and duck (in this study) are marked at the corresponding position. (B) Multiple sequence alignment of partial EDNR receptors. * Indicates amino acid identity. V314M and R332H mutations are indicated in red color.