Figure 3. Loss of p47^phox improves adriamycin-induced matrix deposition.

(A) Masson's Trichrome staining of kidneys from uninjured (Control) or adriamycin-treated (4w-ADR) WT, α1KO, p47^phoxKO, and DKO mice. Note the presence of fibrillar collagen (blue) in both glomeruli and tubules of integrin α1KO mice which was reduced in kidneys of p47^phoxKO and DKO mice. (B) Collagen IV staining of kidney sections from 4 weeks injured WT, α1KO, p47^phoxKO and DKO mice. Note the high levels of glomerular collagen IV in integrin α1KO mice which were reduced in kidneys of p47^phoxKO and DKO mice. (C) Equal amount of kidney lysates (20 μg/lane) from WT, α1KO, p47^phoxKO, and DKO mice (n=3 shown) 4 weeks after adriamycin injection were analyzed by Western blot for levels of collagen IV and collagen I. (D) The collagen I, collagen IV and β-actin bands were analyzed by densitometry analysis and the levels of collagen IV are expressed as collagen I/β-actin or collagen IV/β-actin ratio. The values represent the mean ± SD of the number of kidneys indicated.