Light microscopic localization of ezrin, PKA and CFTR in human salivary glands. A: SMG; ezrin reactivity is present around the lumen and along the intercellular canaliculi in secretory acini (AC), and around the lumen of intercalated duct (ID) cells. Inset: Parotid; ezrin is present along the luminal surface of striated duct cells (SD). B, C: In striated duct cells, RII subunits of PKA are present along the luminal membrane (arrowheads) and in the apical cytoplasm; in some cells reactivity also is present in the basal cytoplasm. In acinar cells, RII is present mainly in the apical region. B, SMG; C, parotid gland. D: SMG; C subunits of PKA are present along the luminal membrane (arrowheads) and in the basal cytoplasm of striated duct cells. E: SMG; CFTR is present along the luminal membrane (arrowheads) of striated duct cells, in the apical cytoplasm, and in the basal cytoplasm of some cells. No reactivity is present in the acini. F: Parotid; CFTR reactivity is present along the luminal membrane (arrowheads) and in the apical cytoplasm of striated duct cells. G: SMG; no labeling is seen in control sections incubated with non-immune IgG instead of primary antibody. Scale bars = 20 μm.