Kidney tissues were harvested 24 h after reperfusion from the sham, vehicle, and Wnt agonist-treated (wntAg) groups. Total lysates were subjected to Western blotting, with representative blots against (A) β-catenin and β-actin, and (B) cyclin D1 and β-actin, shown. Blots were scanned and quantified by densitometry. Band intensity of β-catenin was normalized to the corresponding band intensity of β-actin. The ratio of the sham group is designated as 1 for comparison. Data presented as means ± SEM (n=4–6/group) and compared by one-way ANOVA and SNK method; *P < 0.05 vs. sham; #P < 0.05 vs. vehicle.