Fig 5. Validation and expression analysis of (A) milR-1 and (B) milR-21.
Total RNA from strain P2niaD18, the recipient ∆ku70FRT2 as well as Dicer single and double mutant strains were used for polyacrylamide gel electrophoresis and northern blot analysis. Mature milRNAs (milRs) and milRNA precursors (pre-milRs) were detected with revers complement 32P-labled DNA probes. Below, loading controls of the total RNA, stained with ethidium bromide (EtBr), and the predicted secondary structures of milRNA precursors are given. On the secondary structures, milRNA sequences are highlighted in red and arrows indicate the expected Dicer cleavage sites.