Table 1. Statistical summary of small RNA sequencing data and distribution of small RNAs with perfect match to the genome sequence of P. chrysogenum.
Total reads | Unique reads (≥ 10 reads) | ||||||
---|---|---|---|---|---|---|---|
RNAs (15–36 nt) | RNA-Mix | ∆ku70FRT2 | ∆dcl2∆dcl1 | RNA-Mix | ∆ku70FRT2 | ∆dcl2∆dcl1 | |
Raw reads | 46,240,082 | 52,144,415 | 65,660,252 | 122,018 | 113,603 | 104,922 | |
Mapped reads[ 1 ] | 39,188,594 | 45,134,231 | 57,721,023 | 89,096 | 77,035 | 67,428 | |
Ratio | 84.80% | 86.60% | 87.90% | 73.01% | 67.81% | 64.26% | |
Protein-coding genes | 16,171,567 | 16,249,031 | 20,055,379 | 38,337 | 35,173 | 26,558 | |
sense | 11,612,935 | 10,975,391 | 13,497,944 | 29,659 | 27,835 | 24,798 | |
antisense | 4,942,727 | 5,554,209 | 6,874,230 | 10,389 | 8,631 | 2,679 | |
Exon | 5,619,395 | 6,472,049 | 7,539,001 | 16,527 | 17,680 | 8,431 | |
sense | 1,633,831 | 1,687,351 | 1,557,211 | 9,788 | 11,875 | 7,596 | |
antisense | 4,009,410 | 4,799,719 | 5,984,778 | 7,043 | 6,020 | 865 | |
Intron | 10,569,963 | 9,777,092 | 12,504,834 | 21,702 | 17,439 | 18,022 | |
sense | 9,996,408 | 9,276,048 | 11,918,300 | 19,744 | 15,839 | 17,085 | |
antisense | 905,029 | 741,304 | 873,060 | 3,238 | 2,557 | 1,729 | |
tRNA | 6,128,717 | 6,746,039 | 8,779,724 | 5,062 | 4,133 | 4,363 | |
sense | 6,126,353 | 6,745,245 | 8,779,649 | 5,025 | 4,123 | 4,363 | |
antisense | 2,577 | 971 | 277 | 38 | 11 | 1 | |
rRNA | 22,659,643 | 29,792,848 | 39,201,820 | 43,143 | 34,808 | 39,639 | |
sense | 22,652,991 | 29,780,783 | 39,201,243 | 43,051 | 34,639 | 39,626 | |
antisense | 6,652 | 12,065 | 577 | 92 | 169 | 3 | |
Intergenic | 21,367,882 | 27,127,757 | 35,564,101 | 50,226 | 41,195 | 35,646 |
[1] Perfectly mapped reads with more than one locus may occur in several categories as mapped reads.