Fig 2. GnRH responsive transcripts increase after ovariectomy and decrease after Antide treatment.

Gonadotrope specific transcripts were isolated from female mice either sham operated or OVX and then treated with either vehicle or Antide. Real-time PCR 2^-ΔCt values for five separate experiments (n = 5) were plotted showing the median with interquartile range of total pituitary levels of A.) Lhb; B.) Fshb; C.) Cga; and D.) Gnrhr and gonadotrope levels of E.) Lhb; F.) Fshb; G.) Cga; and H.) Gnrhr. Statistical differences were determined by a one-way ANOVA followed by Tukey’s multiple comparison test (*<0.05; **<0.01; ***<0.001). Each “n” represents 5–7 pooled pituitaries per treatment group.