Fig 4. Activin and inhibin subunit transcript levels are refractory to regulation by ovarian hormones or GnRH.

2^-ΔΔCt values were calculated using total RNA from LβT2 cells as the calibrator. The median with the interquartile range is indicated for total pituitary levels of A.) Inha; B.) Inhba; and C.) Inhbb and gonadotrope levels of D.) Inha; E.) Inhba; and F.) Inhbb. Statistical differences (n = 5) were determined by a one-way ANOVA followed by Tukey’s multiple comparison test (***<0.001). Each “n” represents 5–7 pooled pituitaries per treatment group.