Fig 5. Smad3 mRNA in gonadotropes may be ovarian dependent and GnRH independent.

2^-ΔΔCt values were calculated using total RNA from LβT2 cells as the calibrator and these levels were plotted with the median indicated along with the interquartile range for pituitary A.) Smad2; B.) Smad3; C.) Smad4; and D.) Smad7 and the gonadotrope E.) Smad2; F.) Smad3; G.) Smad4; and H.) Smad7. Statistical differences were determined by a one-way ANOVA followed by Tukey’s multiple comparison test. Here n = 3 or 4, where “n” represents 5–7 pooled pituitaries per treatment group).