Table 1. Transcripts, primer concentrations and primer sequences used in real time analysis.
Transcript | Concentration | 5'→3' |
---|---|---|
Lhb | 300 nM | CACCTTCACCACCAGCATCT |
GCACAGGAGGCAAAGCA | ||
Gnrhr | 300 nM | TTGTCTTTGCAGGACCACAGTTAT |
TGGGTCACACATTGCGAGAA | ||
Cga | 150 nM | TGCTTCTCCAGGGCATATCC |
CTTTGGAACCAGCATTGTCTTCT | ||
Fshb | 300 nM | TGCCGTTTCTGCATAAGCAT |
TCGTATACCAGCTCCTTGAAGGTA | ||
Rpl19 | 150 nM | GGAAAAAGAAGGTCTGGTTGGA |
TGATCTGCTGACGGGAGTTG | ||
Gh | 150 nM | CCCAGGCTGCTTTCTGCTT |
AGCAATTCCATGTCGGTTCTCT | ||
Prl | 300 nM | CCCTGGCTACACCTGAAGACA |
GACTGCACCAAACTGAGGATCA | ||
Tshb | 300 nM | CACCATCTGTGCTGGGTATTGT |
GTGCATATTTGGGAAGAAACAGTTT | ||
Foxl2 | 150 nM | TCCGGCATCTACCAGTACATCA |
TATTCTGCCAGCCCTTCTTGTT | ||
Inhba | 150 nM | AGAAGGCAACCACACGACTTTT |
CTTGCCAACAGAAATCCTCTCA | ||
Inhbb | 300 nM | AGACATCGCATCCGCAAAC |
GATGAAGAACTGTTGCCTGCAA | ||
Inha | 300 nM | TGCACAGGACCTCTGAACCA |
GGCACCTGTAGCTGGGAAAA | ||
Smad2 | 300 nM | TTGTGCAGAGCCCCAACTG |
CGGCTTCAAAACCCTGGTT | ||
Smad3 | 300 nM | CTCTCCCCGAATCCGATGT |
AGGCCGGCTCACAGTAGGT | ||
Smad4 | 150 nM | AGACTACCCCAGGCAGAGCAT |
GCTCGGTGAAGGTGAATCTCA | ||
Smad7 | 300 nM | CCATCAAGGCTTTTGACTATGAGA |
AAGCTGATCTGCACGGTGAA |