Fig. 12.

Effect of UTPγS and RB-2 on glial cell migration. Transwell inserts with 10 μg/mL fibronectin-coated polyester membranes were used to separate two chambers in 24-well dishes. Glial cells from purified cultures with 20–22 days were dissociated and seeded in serum-containing medium (3 × 10⁴ cells/chamber) onto membrane in the upper chamber, whereas the lower chamber contained 20 μM UTPγS or 100 μM RB-2. After 4.5 h at 37 °C, cells at the lower side of the membranes were fixed, stained, and counted in 10 fields along the diameter of the membrane as described in the “Materials and methods” section. a–c Representative micrographs of migrated cells in control, UTPγS-, and RB-2-treated cultures. d Quantification of migrated cells. Data represent the mean ± SEM of four experiments performed in duplicate. Bar = 20 μm