Fig. 4.
Effect of ADP and UTP on the intracellular calcium content of glial cells growing in the scratched area of the cultures. Retinal cultures at E8C7 were scratched, cultivated for 2 days, and loaded with the calcium indicator Fluo3-AM as described in the “Materials and methods” section. a, b Confocal micrographs of glial cells at the border of the scratched area before stimulation with nucleotides. c, d Confocal micrographs of the same glial cells at the border of the scratched areas shown in a and b after stimulation with 100 μM ADP (c) or UTP (d). e, f Representative traces of [Ca2+]I transients, measured as peak heights, after ADP and UTP stimulation (arrows). g, h Intensity of fluorescence before (control) and peak intensity of fluorescence after ADP and UTP stimulation of the cultures. Data in g and h represent the mean ± SEM of fluorescence intensity of 15 and 16 glial cells recorded in three separate experiments. Bar = 30 μm