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The characterization of the wild-type and mutants of P2X4R. a Trace recording of 1–3 mM ATP-induced currents for the Y54A/S, F198A/S, and W259A/S mutants and example records of 10 μM ATP-induced currents in the absence of IVM for the WT, Y195A/S, F200A/S, and F330A/S mutants. b Concentration–response curves of ATP for the WT (n = 15) and the Y195A/S (n = 8/7), F200A/S (n = 6/4), and F330A/S mutants (n = 6/5)
