Fig. 3.

The characterization of the substitution-deficient mutants of P2X₄R. a The expression pattern of WT and Y195A/S, Y54A/S, F198A/S, and W259A/S mutants in HEK293 cells, examined by laser-scanning confocal microscopy (three experiments). b The effects of 3 μM IVM on the 10 μM ATP-induced current in HEK293 cells expressing the F198A/S mutants. c The augmentation of the maximum current amplitude of the WT (n = 15), F198A/S (n = 5/5), and W259A/S (n = 4/4) by 3 μM IVM. d Concentration–response curves of IVM potentiation for the WT (n = 10) and F198A (n = 6) receptor. (e). Concentration–response curves of IVM deactivation for the WT (n = 10) and F198A (n = 6) receptor