Table 2.

IVM potency of wild-type and mutant receptors

Receptors	IVM potentiation EC50 (μM)	nH	IVM deactivation EC50 (μM)	nH	n
WT P2X4	0.23 ± 0.05	1.8 ± 0.2	2.16 ± 0.45	2.1 ± 0.4	10
Y195A	0.78 ± 0.11**	1.7 ± 0.3	1.97 ± 0.24	2.2 ± 0.5	6
Y195S	0.82 ± 0.23*	2.1 ± 0.3	2.12 ± 0.38	2.4 ± 0.4	7
F198A	2.02 ± 0.52**	2.1 ± 0.2	6.12 ± 1.85**	2.3 ± 0.1	6
F198S	1.90 ± 0.44**	1.4 ± 0.4	5.84 ± 1.56*	1.8 ± 0.1	6
F200A	0.02 ± 0.01**	1.1 ± 0.3	4.28 ± 1.88	1.4 ± 0.3	7
F200S	0.03 ± 0.02**	1.2 ± 0.2	4.86 ± 1.62*	1.5 ± 0.3	5
F330A	0.55 ± 0.22	1.8 ± 0.3	1.90 ± 0.44	2.4 ± 0.2	5
F330S	0.63 ± 0.32	1.7 ± 0.4	2.08 ± 0.28	2.1 ± 0.3	6

Each receptor was examined in five to ten cells in the presence IVM, and the number of cells studied is given by n

are denoted with*P < 0.05, or **P < 0.01, respectively, denote significant differences from WT with IVM