Table 3.
Identification of the dominant Campylobacter jejuni strains in the in vivo chicken colonization competition assays by PCR detection of relevant genes
| Group and assay | Sample | Sample type | ars C | vir B11 | cj0144 | tet O | rlo B | cje0256 |
|---|---|---|---|---|---|---|---|---|
| Group1- assay1 | Bird1 | bird feces | + | - | + | + | + | - |
| Group1- assay1 | Bird2 | bird feces | + | - | + | + | + | - |
| Group1- assay1 | Bird3 | bird feces | + | - | + | + | + | - |
| Group1- assay2 | Bird1 | bird feces | + | - | + | + | + | - |
| Group1- assay2 | Bird2 | bird feces | + | - | + | + | + | - |
| Group1- assay2 | Bird3 | bird feces | + | - | + | + | + | - |
| Group1- assay1 + 2 | G2008b | inoculated strain DNA | + | - | + | + | + | - |
| Group1- assay1 + 2 | B2008c | inoculated strain DNA | - | + | - | - | - | - |
| Group2- assay1 | Bird1 | bird feces | + | - | + | - | + | - |
| Group2- assay1 | Bird2 | bird feces | + | - | + | - | + | - |
| Group2- assay1 | Bird3 | bird feces | + | - | + | - | + | - |
| Group2- assay2 | Bird1 | bird feces | + | - | + | - | + | - |
| Group2- assay2 | Bird2 | bird feces | + | - | + | - | + | - |
| Group2- assay2 | Bird3 | bird feces | + | - | + | - | + | - |
| Group2- assay1 + 2 | A2008a | inoculated strain DNA | + | - | + | - | + | - |
| Group2- assay1 + 2 | B2008b | inoculated strain DNA | - | + | - | - | - | - |
| Group3- assay1 | Bird1 | bird feces | - | - | + | + | - | - |
| Group3- assay1 | Bird2 | bird feces | - | - | + | + | - | - |
| Group3- assay1 | Bird3 | bird feces | - | - | + | + | - | - |
| Group3- assay2 | Bird1 | bird feces | + (low) | - | + | + | - | - |
| Group3- assay2 | Bird2 | bird feces | + (low) | - | + | + | - | - |
| Group3- assay2 | Bird3 | bird feces | - | - | + | + | - | - |
| Group3- assay1 + 2 | F2008d | inoculated strain DNA | - | - | + | + | - | - |
| Group3- assay1 + 2 | D2008a | inoculated strain DNA | - | - | - | + | - | + |
Detection of genes specific to the inoculated strains in the in vivo competition assays seven days post-inoculation; (+) gene detected in the sample; (−) gene not detected in the sample; (low) weak signal observed.