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. 2015 May 5;16:30. doi: 10.1186/s12868-015-0159-1

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© Okada et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Blocking Kir2.1 current increased the titer of Lv-synapsinP-Kir2.1. (A and B) The titer of Lv-synapsinP-Kir2.1 was increased by blocking the Kir2.1 current. The viral vector was prepared in the presence or absence of 0.3 mM BaCl₂. The titers of unconcentrated (A) and concentrated (B) viral vectors were measured in host PC12 cells. The addition of Ba²⁺ significantly increased the titers (*p < 0.05, **p < 0.005, Student’s t test, n = 4).