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. 2015 Apr 8;15:23. doi: 10.1186/s12896-015-0136-x

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© Yang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Production of ROS by neutrophils stimulated with recombinant HP-NAP determined by luminol-dependent chemiluminescence assay. A, Human neutrophils (1 x 10⁵ cells) were treated with 0.5 μM HP-NAP and D-PBS, pH 7.2, as a negative control at 37°C. The content of ROS generated from neutrophils was measured continuously by using luminol-dependent chemiluminescence assay as described in Methods. B, The integral chemiluminescence signals calculated from the area under curve and the peak value of chemiluminescence responses of neutrophils stimulated with HP-NAP were used to compare with the control. Data were represented as the mean ± S.D. of one experiment in triplicate (*, p<0.05). Similar results were obtained in two independent experiments.