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. 2015 Mar 12;6(1):22. doi: 10.1186/s13287-015-0023-3

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© Su et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vitro osteogenesis of periodontal ligament stem cells. (A) Cells grown in osteogenic media stained positively for alkaline phosphatase (ALP) (A) and Alizarin red (B) after 28 days of culture and for osteocalcin (C) and type I collagen (D) using immunocytochemistry after 21 days of culture. (E) ALP activities in osteogenic condition increased significantly at day 7 when compared with that at day 3, but there was no significant difference in ALP activities at days 3 and 7 in the control group. *P <0.05 versus control group. Magnifications: 100× (A, C) and 200× (B, D). OD, optical density.