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. 2015 Apr 11;15:24. doi: 10.1186/s12896-015-0140-1

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© Portevin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Whole-cell MALDI-TOF can monitor immune activation events. Vent diagrams depicting for A) Pan-monocytes or B) CD16+ monocytes, m/z peak intensities significantly different between the detailed culture conditions (two-way ANOVA statistical analysis, p < 0.001). C) Smoothed mass spectra quadruplicates and respective scatter-plot of quadruplicate’s averaged mass peak intensities from resting CD16⁺ monocytes (green overlay) and LPS-treated CD16⁺ monocytes (red overlay) highlighting m/z areas containing putative phosphorylation of m/z 11316.24 into m/z 11396.28 (two-way ANOVA with Bonferroni post-tests correction, *** p < 0.001).