Table 7.
Validation of Affymetrix array data with qPCR. Relative transcripts levels in qPCR (mean ± SEM) were measured in the linear range of fluorescence versus cycle curve
| Gene | 8 weeks | 24 weeks | qPCR | Microarray | Fold change | P |
|---|---|---|---|---|---|---|
| n = 4 | n = 5 | Fold change | P | |||
| Afp | 2.25 ± 0.99 | 0.36 ± 0.33 | −6.3 | 0.086 | −10 | 0.01 |
| Apob | 2.03 ± 0.60 | 0.31 ± 0.29 | −6.5 | 0.028 | −8.8 | 0.02 |
| Apoh | 1.77 ± 0.48 | 0.37 ± 0.36 | −4.8 | 0.047 | −6.9 | 0.02 |
| Ceacam11 | 1.77 ± 0.80 | 0.40 ± 0.24 | −4.4 | 0.113 | −4.8 | 0.01 |
| Apom | 2.08 ± 0.95 | 0.27 ± 0.26 | −7.6 | 0.080 | −4.2 | 0.02 |
| Ttr | 2.23 ± 1.25 | 0.18 ± 0.16 | −12.7 | 0.008# | −4.3 | 0.03 |
| Nrk | 1.54 ± 0.32 | 0.36 ± 0.25 | −4.3 | 0.020 | −3.9 | 0.01 |
| Pramef12 | 1.63 ± 0.09 | 0.40 ± 0.17 | −4.0 | 0.001 | −3.7 | 0.002 |
| Slc12a1 | 1.79 ± 0.41 | 0.34 ± 0.25 | −5.3 | 0.016 | −3.3 | 0.02 |
| Scgb1a1 | 0.21 ± 0.03 | 1.72 ± 0.42 | 8.4 | 0.001# | 5.1 | 0.0001 |
| Fxdy3 | 0.40 ± 0.11 | 1.42 ± 0.59 | 3.6 | 0.172 | 3 | 0.004 |
| Gabrp | 0.53 ± 0.23 | 1.31 ± 0.26 | 2.5 | 0.066 | 2.6 | 0.001 |
| Clic6 | 0.26 ± 0.15 | 1.59 ± 0.66 | 6.1 | 0.123 | 2.2 | 0.02 |
| Serpina3n | 0.07 ± 0.01 | 0.16 ± 0.04 | 2.3 | 0.121 | 2.2 | 0.0002 |
| Cxcl6 | 0.71 ± 0.28 | 1.37 ± 0.21 | 1.9 | 0.092 | 2.2 | 0.01 |
| Krt85 | 0.51 ± 0.09 | 1.50 ± 0.42 | 3.0 | 0.045# | 2 | 0.01 |
| Tmprss11g | 0.54 ± 0.10 | 1.38 ± 0.51 | 2.5 | 0.196 | 2.1 | 0.02 |
| Cd79a | 0.39 ± 0.24 | 1.57 ± 0.23 | 4.0 | 0.009 | 2 | 0.0002 |
| Housekeeping gene | ||||||
| Cyclophilin | 0.13 ± 0.01 | 0.14 ± 0.03 | 0.949 | |||
Comparison of qPCR was by t-test, those indicated by # were log transformed to normalize variances.