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. 2015 Apr 6;3(4):e12355. doi: 10.14814/phy2.12355

Figure 4.

Figure 4

Effect of lactobacilli on heat shock protein (HSP) 27 expression in porcine IPEC-J2 epithelial cells. (A) Cells grown on six-well tissue culture plates and reached a confluent layer were left untreated or treated with bacterial cells of LGG, Lactobacillus johnsonii, and Lactobacillus reuteri at multiplicity of infection (MOI) of 100 for 6 h, followed by a 6-h recovery. (B) Results from stimulation with supernatants from LGG, L. johnsonii, and L. reuteri (sLGG, sLj, and sLr). MRS broth was used as a vehicle control. In all experiments, the heat shock treatment, where cells were heat treated for 60 min at 42°C followed by a 2-h recovery, served as a positive control. Densitometry of HSP27 expression was calculated using heat shock cognate protein (HSC) 73 as equal protein loading control. Images shown represent three separate experiments. Data given are means (±SEM). Values with different letters differ significantly (P < 0.001).