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. 2015 Apr 6;3(4):e12355. doi: 10.14814/phy2.12355

Figure 5.

Figure 5

Effect of lactobacilli on heat shock protein (HSP) 72 expression in porcine IPEC-J2 epithelial cells. (A) Cells grown on six-well tissue culture plates and reached a confluent layer were left untreated or treated with bacterial cells of LGG, Lactobacillus johnsonii, and Lactobacillus reuteri at multiplicity of infection (MOI) of 100 for 6 h, followed by a 6-h recovery. (B) Results from stimulation with supernatants from LGG, L. johnsonii, and L. reuteri (sLGG, sLj, and sLr). MRS broth was used as a vehicle control. In all experiments, the heat shock treatment, where cells were heat treated for 60 min at 42°C followed by a 2-h recovery, served as a positive control. Densitometry of HSP72 expression was calculated using heat shock cognate protein (HSC) 73 as equal protein loading control. Images shown represent three separate experiments. Data are given as means (±SEM). Values with different letters differ significantly (P < 0.001).