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. 2015 Apr 6;3(4):e12355. doi: 10.14814/phy2.12355

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of bacteria on transepithelial electrical resistance (TEER) in IPEC-J2 cells grown on transwell filters. (A) Polarized cells left untreated or treated with bacterial cells of LGG, Lactobacillus johnsonii, and Lactobacillus reuteri at multiplicity of infection (MOI) of 100 for 6 h or ETEC (MOI 10) for 3 h. (B) First hour in which IPEC-J2 cells were challenged by ETEC (MOI 10) after being preincubated with Lactobacillus spp. (C) Last hour (hour 3) in which IPEC-J2 cells were challenged by ETEC (MOI 10) after being preincubated with Lactobacillus spp. TEER values are expressed in Ω cm². The controls showing higher values than 100% because the percentage of TEER changes during incubation with bacteria was calculated based on the TEER values of control cells at h 0. Data given are means (±SEM) of at least four separate experiments. Values with different letters differ significantly (P < 0.001).