. 2015 Apr 22;3(4):e12364. doi: 10.14814/phy2.12364

Table 1.

SYBR Green primers set sequences

Target mRNA	Strand	Primer Sequence (5′ → 3′)	Amplicon size (bp)
PGC-1α total	Forward	cagcctctttgcccagatctt	99
PGC-1α total	Reverse	gtggactcaagtggtgcagt	99
PGC-1α-ex1a	Forward	atggagtgacatcgagtgtgct	127
PGC-1α-ex1a	Reverse	acagctttctgggtggactc	127
PGC-1α-ex1b	Forward	tcacaccaaacccacagaga	61
PGC-1α-ex1b	Reverse	ctggaagacatgatacac	61
IGF-1	Forward	ggctgaccaagctgaaactc	176
IGF-1	Reverse	acctcctgggtttaagcgat	176
MGF	Forward	ggctgaccaagctgaaactc	176
MGF	Reverse	acctcctgggtttaagcgat	176

PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; IGF-1, insulin-like growth factor 1; MGF, mechano-growth factor; bp, base pair.