Table 2.
mRNA expression of reference genes in insulin-sensitive tissues from C57BL/6J mice
| Gene | EDL | Soleus | Liver | Subcutaneous | Epididymal |
|---|---|---|---|---|---|
| Rn18S | NA | 9.66 ± 0.06 | NA | 8.83 ± 0.12 | NA |
| Actb | NA | 23.84 ± 0.07 | 21.95 ± 0.10 | 18.18 ± 0.23 | NA |
| B2m | 24.65 ± 0.12 | 24.61 ± 0.08 | 20.25 ± 0.08 | 19.86 ± 0.14 | NA |
| Gapdh | NA | 19.79 ± 0.05 | NA | 20.56 ± 0.15 | 19.47 ± 0.14 |
| Gusb | 29.36 ± 0.12 | 29.17 ± 0.09 | NA | 26.49 ± 0.12 | NA |
| Hprt1 | 27.58 ± 0.07 | 27.86 ± 0.07 | NA | 25.45 ± 0.12 | 24.93 ± 0.10 |
| Pgk1 | 22.88 ± 0.09 | 25.14 ± 0.08 | 25.09 ± 0.07 | 25.51 ± 0.11 | 26.20 ± 0.11 |
| Ppia | 24.93 ± 0.08 | 25.61 ± 0.06 | 21.44 ± 0.09 | 21.93 ± 0.12 | 20.92 ± 0.11 |
| Rplp0 | 22.35 ± 0.06 | 21.98 ± 0.10 | NA | 20.25 ± 0.20 | NA |
| Tbp | NA | 31.56 ± 0.10 | NA | 30.85 ± 0.15 | NA |
Data are presented as Cq values and expressed as mean ± SEM. NA, not applicable.