Figure 2.

Dependence of fluorescence quenching on Ca²⁺ concentration for CaM-AF488 (200 nM) in the presence of S1179D-eNOS (800 nM) at Ca²⁺ concentrations from near zero to 6 μM. The Ca²⁺ concentration noted in each panel was generated by adding various amounts of a high-Ca²⁺ buffer containing 10 mM Ca²⁺ to a low-Ca²⁺ buffer containing 2 mM EGTA. Ca²⁺ concentrations were calibrated as described in Methods.