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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Exp Eye Res. 2015 Mar 19;134:33–38. doi: 10.1016/j.exer.2015.03.016

Figure 2.

Figure 2

Representative immunohistochemistry for nidogen-2 in human corneas. Column A. Nidogen-2 protein was noted in the epithelial basement membrane (arrows) and epithelium (e), as well as at low levels in keratocytes (arrowheads) in unwounded control corneas. Column B. Negative control in unwounded cornea with isotypic control antibody showed no non-specific immunostaining for nidogen-2. Column C. In a cornea at 30 minutes after epithelial scrape, nidogen-2 protein was up-regulated in stromal keratocytes (arrowheads), including anterior stromal keratocytes in the early stages of apoptosis detected with the TUNEL assay (not shown). Staining near the stromal surface (arrows) could represent BM that was not completely removed by scrape or expression in keratocytes undergoing apoptosis. Column D. Isotypic control shows specific antigen-antibody interaction in the control cornea after epithelial scrape. Blue is DAPI staining of cell nuclei. e is epithelium. Magnification 400x.