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. Author manuscript; available in PMC: 2016 May 8.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Mar 20;460(3):609–615. doi: 10.1016/j.bbrc.2015.03.077

Fig. 1.

Fig. 1

Chk1 phosphorylation and a checkpoint protein complex assembly in response to MMC-induced ICLs in Xenopus egg extract. (A) Caffeine or KU55933 was incubated in egg extracts supplemented with sperm chromatin and mitomycin C (MMC). After 1-hr incubation, Chk1 phosphorylation (Chk1 P-S344) and total Chk1 in extracts were examined via immunoblotting analysis. (B) VE-822 or NU6027 was incubated in egg extracts supplemented with sperm chromatin and MMC. After 1-hr incubation, Chk1 phosphorylation and total Chk1 in extracts were examined via immunoblotting analysis. (C) MMC or aphidicolin (APH) was incubated in egg extracts supplemented with sperm chromatin. After 1-hr incubation, chromatin fractions (“chromatin”) and total extract (“extract”) were isolated and examined via immunoblotting analysis as indicated. Orc2 was used as a loading control.