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. Author manuscript; available in PMC: 2016 May 8.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Mar 20;460(3):609–615. doi: 10.1016/j.bbrc.2015.03.077

Fig. 3.

Fig. 3

REV1 is important for Chk1 phosphorylation in response to MMC-induced ICLs and stalled DNA replication forks. (A) MMC was incubated in mock-depleted or REV1-depleted egg extracts supplemented with sperm chromatin. After 1-hr incubation, chromatin fractions and total extracts were examined via immunoblotting analysis as indicated. (B) APH was incubated in mock-depleted or REV1-depleted egg extracts supplemented with sperm chromatin. After 1-hr incubation, chromatin fractions and total extracts were analyzed via immunoblotting analysis. Histone 3 was used as a loading control.