Table 1.

PCR primer sequences and expected sizes of resulting amplicons for BEST1 sequencing.

Target	Forward Primer / Reverse Primer	Amplicon Size (bp)
exon 1	TCAGTGTACCTCCCTGTCTGT/ TGGCTATTTGTTCCAAGACCC	282
exon 2	CAAAGAGGCAGCCACACTCCC/ CAGCCCTGAACTGCCGAGA	282
exon 3	CGCGCTCCGGTCAAGGGAGATG/ GGTCCTTCGCCCGTCTGAAGCC	387
exon 4	ATCCCGGCCACGCTGCCAA/ TCTACCCAGAGCACAACCGTCCC	292
exon 5	GTGGTCAGAACCCCAACCCTC/ AAGTGAGCCCTTCCCATCACA	201
exon 6	TCACAGAGGGCCAAAGTCAC/ TCAGCCATGACCCTTCTAAAGC	336
exon 7	AACAGAAGTTTAAGGGCAAGG/ CACAGGACAGGCTCCGTCTC	188
exon 8	CTTGGGCTGACTTTTCTGTTGGA/ CTTTCCTAAGTCCTTGCCCTTG	264
exon 9 (5′)	GTCCTGCTTTGGTCCTTTGTCC/ GCAGCAGTCTCAAAAGCACCC	310
exon 9 (center)	GCCAGCCCAAGAAACTCGG/ ACTCGACAGTTTTCCTCTTCACG	342
exon 9 (3′)	AGACCAGTTCTGAAGCACTCC/ TGGGTGTAAATCCAGCATCCTC	386
exon 10	TCCAAGGTCCCCAAGTATGTCT/ TTTGACCACAGAAGTGACTGC	294
exon 11	AACCTTTGCCCTAACTCCC/ TCCAGTGGTTGATTCCAGC	338