(A) The experimental paradigm in vivo. (B) Continuous ECoG recordings show that ICV albumin does not induce acute seizures or status epilepticus (common in other models of acquired epilepsy, e.g pilocarpine), yet results in latent appearance of spontaneous seizures. (C) Representative seizure recorded 72 h after pump implantation. (D) Comparison of number of seizures per day (averaged over 3 day periods) shows higher incidence of seizures in the albumin-infused mice (black line, n=13), compared to controls (red line, n=10). Notably, seizures appeared 3 days after initiating albumin infusion, and lasted well after the extraction of the pumps on day 7. Error bars indicate SEM.